Bloody Minded - Episode 13 - In the Lab: Dr Coombs? Who is DAT? | Direct Coombs Test

Episode 13 - In the Lab: Dr Coombs? Who is DAT? | Direct Coombs Test

08/20/24 • 30 min

“It’s Test. Coombs Test. And I like my erythrocytes shaken, not stirred.” Return the lab (where some of the Bloody Minded Crew truly belong) and uncover the secrets of the most important test in haemolysis - the Direct Antiglobulin Test. Learn to whisper its secrets and it can guide you to identifying the true culprit behind these broken red cells.
Timestamps:
0:57 - Coombs Noir
2:05 - Naming History
4:32 - DAT in the Lab
11:52 - Doing a DAT - Exam Version
14:35 - Eluates (Briefly)
15:45 - False Positives & Negatives
18:24 - When to DAT?
21:55 - Distilling the DAT - Interpretation
25:38 - DAT-Vanced Topics
29:24 - Takehome Points

Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

Previous Episode

Episode 12 - Haemolysis: Breaking it down! | Testing for Haemolysis

Haemolysis? Do I really hapto send off more tests? This is reticulous! Now you know what a red cell is - lets learn about how they can break down. Join us as we explore what your boss actually wanted you to do when they asked for a haemolysis screen, and about Cale's deep seated distrust of hepatologists.
Timestamps
2:30 - Haemolysis - what is it (good for?)
7:58 - Reticulocytes? What a reticulous name for a cell
9:36 - LDH - Nick will talk about biochemistry here, I'm SORRY okay?
11:36 - Bilirubin feat. Gilbert - winner of the 2015 Triple J's Hottest 100
16:10 - Haptoglobin (with apologies to my anaesthetist friends)
20:32 - Who is DAT?
23:49 - Haemolysis screen recap - click here when med reg is impatient
27:35 - Take home messages!

Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

Next Episode

Episode 14 - Throwing Frags | Introduction to MAHA

Feel your knowledge fragmenting? A MAHA doesn't make you haha? I mean, that's probably a good thing - they're quite serious. Time to unpack the troublesome schistocytes. Hope you brought your helmets as we teach you the bare minimum about fragments so you can save lives on the ward.
Timestamps
02:26 - What is a red cell fragment?
06:09 - How are schistocytes born?
09:40 - DIC. The haem one, we mean.
13:32 - TTP or not TTP
16:15 - Sorry, he's not single
19:02 - HUS - a harsh renality
20:19 - aHUS - complements incoming
23:14 Take home messages

Check out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au