Episode 08 - Identification of ACE2 modifiers by CRISPR screening

This week we discuss high throughput CRISPR/Cas9 screening and how these have been adapted to investigate novel treatments for COVID-19 with Emily Sherman a former Post Doc and Brian Emmer (@brianemmer), a new PI at University of Michigan (@UMIntMed)

In this episode we cover details on how to conduct a high throughput CRISPR/Cas9 screen, the exciting advances in NextGen sequencing as well as what it's like to set up your own lab while working as a clinician during a pandemic. We also speak to Emily about her transition from academia to industry and our usual discussion about the benefits of preprints.

Read the full preprint - https://www.biorxiv.org/content/10.1101/2021.06.10.447768v1.full

Brian’s lab homepage (https://emmer.lab.medicine.umich.edu/)

https://www.nature.com/articles/nm1267

This episode was produced by Emma Wilson and edited by Jonny Coates and John D Howard.

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Produced by JEmJ Productions (find us on Twitter: Jonny @JACoates, Emma @ELWilson92, John @JohnDHoward8) and generously supported by ASAPbio (https://asapbio.org | @asapbio_).