Mentors at Your Benchside - Multiple Fragment Ligation: The Why and How

Multiple Fragment Ligation: The Why and How

05/14/24 • 7 min

#105 — You may be familiar with standard single fragment ligations: insert, vector, ligase—done! But what if you have a complex cloning project with a massive region of DNA to clone? You can’t PCR the whole thing, and you can’t cut the entire thing out from somewhere else. What do you do?

In this episode, we explain the answer: multiple fragment ligation.

Check out the corresponding article for some handy illustrations and links to related resources. [1] To discover more ways to go about molecular cloning, check out these five approaches. [2] And read this article to dive deeper into how ligation works. [3]

Resources:
1. Multiple Fragment Ligation: The Why and How. Available at: https://bitesizebio.com/67124/multiple-fragment-ligation/
2. Cloning Methods: 5 Different Ways to Assemble. Available at: https://bitesizebio.com/26961/cloning-methods-5-different-ways-to-assemble/
3. DNA Ligation: How it Works & 6 Top Tips. Available at: https://bitesizebio.com/10279/how-dna-ligation-works/

In this episode, we explain the answer: multiple fragment ligation.

Previous Episode

Funding Opportunities and the Flow of Money in Science

#104 — What funding stream is right for you? Industry or government? Non-profits or crowdfunding? It depends on what you're researching, but also where you want to take your career.

In this episode, Joel Berry, Founder, and Chief Scientist at Astound Research, breaks down the different funding streams and flow of money in bioscience research. Discover the scope and requirements of each type of funding source, and get a breakdown of the overall funding landscape so you can pick the funding stream that works best for you.

Check out the corresponding online article for links to more helpful funding resources. [1] Plus, discover how you can advocate for science funding [2] and dive deeper into whether crowdfunding will work for your project. [3]

Resources:
1. Funding Opportunities and the Flow of Money in Science. Available at: https://bitesizebio.com/77353/funding-opportunities-landscape/
2. Defend Science Funding! A Brief Guide. Available at: https://bitesizebio.com/36701/defend-science-funding/
3. Kick Start Your Research With Crowdfunding! Available at: https://bitesizebio.com/20876/kick-start-your-research-with-crowdfunding/

Next Episode

Tissue Processing For Histology: What Exactly Happens?

#106 — Transforming a tissue sample into a slide ready for microscopic exploration involves a series of critical steps. Among these, tissue processing is a fundamental phase bridging tissue fixation and the embedding/sectioning of paraffin blocks.

In this episode, discover what exactly happens in this vital in-between stage, and learn about the six steps that ensure your samples are ready for microscopic examination. [1]

And while you're here, check out our related articles on tissue fixation and embedding/sectioning [2,3], and the five important stages in histology slide preparation. [4]

Resources:
1. Tissue Processing For Histology: What Exactly Happens? Available at: https://bitesizebio.com/13469/tissue-processing-for-histology-what-exactly-happens/
2. An Introduction To Fixation For Histology: Think Before You Fix! Available at: https://bitesizebio.com/13401/think-before-you-fix/
3. Tissue Embedding and Sectioning: Something to Think About Whilst in the Bath. Available at: https://bitesizebio.com/13414/tissue-embedding-and-sectioning-something-to-think-about-whilst-in-the-bath/
4. How Histology Slides Are Prepared. Available at: https://bitesizebio.com/13398/how-histology-slides-are-prepared/