ID:IOTS - Infectious Disease Insight Of Two Specialists

Back after a summer break, Jame and Callum discuss the fascinating machine that is the MALDI-TOF. Matrix Assisted Laser Desorption Ionisation Time Of Flight mass spectrometry. We discuss the basics of how it functions, its application in the lab and some pros and cons of its use along with what the future may hold. Here are some relevant videos and papers:
- Exciting video of MALDI-TOF
https://www.youtube.com/watch?v=0jeFpXHZ8W0&ab_channel=BrukerDaltonics
- MALDI-TOF to detect MRSA https://www.frontiersin.org/articles/10.3389/fmicb.2020.00232/full
- Direct MALDI-TOF on blood cultures
https://www.spandidos-publications.com/10.3892/etm.2020.9365
- MALDI-TOF to detect CPE
https://www.frontiersin.org/articles/10.3389/fmicb.2021.789731/full

Send us a text

Support the show

Questions, comments, suggestions to [email protected] or on X/Threads @IDiots_pod
Prep notes for completed episodes can be found here (Not all episodes have prep notes).
If you are enjoying the podcast please leave a review on your preferred podcast app!
Feel like giving back? Donations of caffeine gratefully received!
https://www.buymeacoffee.com/idiotspod

Join Jame and Callum and they discuss some of the other Enterobacterales Gram negatives. Particularly breaking down them into their families and into Lactose fermenters and non-fermenters. Jame has a small breakdown around our Twitter handle near the end.
Comments suggestions and criticisms to [email protected] or @IDiots_pod
Some lists:
Chromosomal AmpC Carriers (high-risk): HECK YES

· Hafnia alvei, Enterobacter cloacae, Citrobacter freundii, Klebsiella aerogenes, Yersinia enterocolitica

Lower Risk Chromosomal AMPC: Vulgar Providencia Store AMPC

· Proteus Vulgaris, Providencia, Serratia, Acinetobacter, Morganella, Providencia

Intrinsic Colistin Resistance:

· Burkholderia cepacia (Nonfermenter), Proteus, Providencia, Serratia

Biochemical mimics of Salmonella (also can group)

· Hafnia, Morganella,Proteus

Cat 3 organisms:

· Salmonella typhi/paratyphi, Shigella dysenteriae type 1, E.coli O157, Yersinia Pestis

Some useful resources:
SMI ID 16: identification of Enterobacteriaceae

https://www.gov.uk/government/publications/smi-id-16-identification-of-enterobacteriaceae

Janda JM, Abbott SL. 2021. The changing face of the family Enterobacteriaceae (order: “Enterobacterales”): new members, taxonomic issues, geographic expansion, and new diseases and disease syndromes. Clin Microbiol Rev 34:e00174-20.

https://doi.org/10.1128/CMR.00174-20

Send us a text

Support the show

Questions, comments, suggestions to [email protected] or on X/Threads @IDiots_pod
Prep notes for completed episodes can be found here (Not all episodes have prep notes).
If you are enjoying the podcast please leave a review on your preferred podcast app!
Feel like giving back? Donations of caffeine gratefully received!
https://www.buymeacoffee.com/idiotspod

Jame and Callum have put this off for far too long... we start exploring the Gram negative bacilli by considering Enterobacterales in general and then discussing E. coli. We focus particularly on the gastrointestinal syndromes causes by different pathogenic strains of E. coli. Did you ever wonder what "coliforms" on a culture result meant - hopefully now you will find out!

Send us a text

Support the show

Questions, comments, suggestions to [email protected] or on X/Threads @IDiots_pod
Prep notes for completed episodes can be found here (Not all episodes have prep notes).
If you are enjoying the podcast please leave a review on your preferred podcast app!
Feel like giving back? Donations of caffeine gratefully received!
https://www.buymeacoffee.com/idiotspod

In this episode Jame and Callum discuss other Beta-lactam antibiotics: aztreonam, temocillin and pivmecillinam... with some substantial tangents into C. difficle and carbapenems.
Send suggestions to [email protected]

Send us a text

Support the show

Questions, comments, suggestions to [email protected] or on X/Threads @IDiots_pod
Prep notes for completed episodes can be found here (Not all episodes have prep notes).
If you are enjoying the podcast please leave a review on your preferred podcast app!
Feel like giving back? Donations of caffeine gratefully received!
https://www.buymeacoffee.com/idiotspod

The last Pharmacokinetics episode had finally been ...Excreted!
This episode covers the ‘drug out’ bit of pharmacokinetics, including things such as:
-What is clearance?
-how to calculate half life
and
-doesn’t everything leave through the kidneys anyway? (That is sort of true, also shut up).
Check out our prep notes (including for this episode) here.

Send us a text

Support the show

Questions, comments, suggestions to [email protected] or on X/Threads @IDiots_pod
Prep notes for completed episodes can be found here (Not all episodes have prep notes).
If you are enjoying the podcast please leave a review on your preferred podcast app!
Feel like giving back? Donations of caffeine gratefully received!
https://www.buymeacoffee.com/idiotspod