
Cannabis Tissue Culture Master Class w/ Dr. Sma Zobayed at Segra International (Podcast Episode 11)
Explicit content warning
03/12/21 • 79 min
Table of Content
0:00 Opening
1:40 Background on Dr. Sma Zobayed
8:41 Responsibilities at Segra International
16:40 Day-To-Day for Dr. Sma Zobayed
30:28 History of Tissue Culture
37:43 Why Tissue Culture?
43:48 Packaging with Green Vault Systems
44:33 Disease Resistance with Tissue Culture
49:29 Plant Count using Tissue Culture
58:03 Differences Between Cuttings from Tissue Culture
1:03:09 Packaging ROI with Precision Batch by Green Vault Systems
1:04:34 The Future of Tissue Culture
1:11:13 Future Expansion/Projects at Segra International
Questions and Topics in this interview:
-Hello, please tell us a bit about yourself
-Let’s talk about your role as “Chief Tissue Culture Officer” at Segra Intl. -
You’re THE expert in tissue culture - what is it? Same as “micropropagation?”
-More and more cannabis growers are turning to tissue culture, what’s the advantage of tissue culture in a commercial grow?
-It seems like a lot of the cannabis enthusiasts I talk to are a bit wary of science and call anything not “naturally” grown “frankenweed”
-What are your predictions for the future of tissue culture in the cannabis space?
-Do you have any new projects or plans you can tell us about?
Table of Content
0:00 Opening
1:40 Background on Dr. Sma Zobayed
8:41 Responsibilities at Segra International
16:40 Day-To-Day for Dr. Sma Zobayed
30:28 History of Tissue Culture
37:43 Why Tissue Culture?
43:48 Packaging with Green Vault Systems
44:33 Disease Resistance with Tissue Culture
49:29 Plant Count using Tissue Culture
58:03 Differences Between Cuttings from Tissue Culture
1:03:09 Packaging ROI with Precision Batch by Green Vault Systems
1:04:34 The Future of Tissue Culture
1:11:13 Future Expansion/Projects at Segra International
Questions and Topics in this interview:
-Hello, please tell us a bit about yourself
-Let’s talk about your role as “Chief Tissue Culture Officer” at Segra Intl. -
You’re THE expert in tissue culture - what is it? Same as “micropropagation?”
-More and more cannabis growers are turning to tissue culture, what’s the advantage of tissue culture in a commercial grow?
-It seems like a lot of the cannabis enthusiasts I talk to are a bit wary of science and call anything not “naturally” grown “frankenweed”
-What are your predictions for the future of tissue culture in the cannabis space?
-Do you have any new projects or plans you can tell us about?
Previous Episode

Scaling From 500 Clones A Week to 100k Clones a Month at 'GlassHouse Farms' (Podcast Episode 10)
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Table of Contents
0:00 Opening
1:53 Catching up w/ Graham since Episode 1
14:24 Cloning at GlassHouse Farms
18:46 Cannabis Compliance
27:01 Tom's Tumbler Batch Machine for Post-Harvest (Python 400)
27:50 Sustainability & Philosophy
35:13 200 Acre Outdoor Hemp Farm
40:33 Future of Cannabis Cultivation in California
48:50 Protecting your Eyes with Method Seven
50:04 Canna Cribs Experience
57:22 Cultivars & R&D at GlassHouse Farms
Questions and Topics in this interview:
-What brought you to the cannabis space?
-Any major changes at Glass House since your episode?
-Are you working with any other breeders?
-In your episode you mentioned you were taking about 500 clones a week, has that increased since my team was there?
-Let’s talk about compliance, in your episode you mentioned that Glass House is at “the forefront of compliant cannabis.” What did that mean at the time?
-You mentioned in your episode that GlassHouse is committed to Sustainable Resource Management, is that throughout the entire process?
-Glass House is obviously a reference to your greenhouse grow style. What are the advantages to growing in a greenhouse over indoor and outdoor grows?
-What did you think about your Canna Cribs experience?
-What would you say to the hand-trim purists?
-We didn’t really talk about extracts when we were at Glass House, but you did mention people were “lining up for” your trim. Is this still the case?
-What were you most excited about showing off in your episode?
-What is your favorite thing about Glass House?
Next Episode

Canna Cribs Podcast #12 - George Murray of Ventana Plant Science
Links:
GH Pro Desk: https://growershouse.com/commercial-division-large-scale-growing-operations
Ventana Plant Science: https://ventanaplant.science/
Shop Ventana Plant Science: https://growershouse.com/ventana-plant-science/?aff=2
Commercial Nutrient Consultations: https://growers.typeform.com/to/cNa0dXPP
George's Blog:https://www.foliarpak.com/category/blog/
_______________________________________________________________________________
Table of Contents
00:00 Opening
00:27 Nick Morin Intro
01:56 George’s Origin Story with Ventana Plant Sciences
08:21 How would you categorize your main area of expertise?
12:20 Approaches to different grow styles (row crop, greenhouse, indoor)
18:20 What is unique to cannabis growing?
21:27 The state of cannabis nutrients currently
25:50 Impact of nutrient runoff in communities
30:10 Growers House Pro Desk
31:12 Mitigating environmental concerns
34:20 Problems with some other nutrients on the market
45:05 DIY nutrient advice
48:10 George’s work with Amino Acids
55:14 Research & Results
59:00 Actionable lessons for growers
01:00:40 Important info for growers
01:08:06 Getting growers comfortable with science
01:12:55 Innovations in plant nutrition
01:24:22 Step by step guide to developing a plant nutrition program
01:32:43 Nutrient spend based on three different models
01:50:10 Best place to find Ventana Plant Science products
__________________________________________________________________
Questions from the Interview
1. How did you get interested in plant nutrition?
2. How long have you been working in the agrochemical industry?
3. What are your main areas of expertise?
4. How much of your work is in the cannabis space versus other areas of agriculture?
5. Can you talk about some of the different grow styles you’ve worked in?
6. How are the nutrient concerns similar/different in these different styles?
Requirements?
7. Runoff/pollution concerns?
8. Can you say how much these different styles might impact the local environment?
9. How to mitigate?
10. What kind of problems do you see with existing nutrients?
11. What kinds of mistakes do people make mixing their own nutrients?
12. I’m interested in your work with different amino acids and mineral interaction. Can you explain this for the layman?
13. What kind of metabolic processes are you looking at?
14. What are some interesting results in this type of work?
15. What lessons can growers take from this information?
16. What is the most important thing any grower might need to know/practice with regard to nutritional programs for plants?
17. Are there any novel or innovative nutritional programs specifically affecting the cannabis industry?
18. What other kinds of innovations are we seeing in plant nutrition as a whole?
19. What can you tell us about ammonium-based nutrients?
20. What are the biggest challenges for growers in your experience?
What could they be doing better?
21. What considerations should be made by growers when developing a plant nutrition program?
22.Do you see a lot of problems with nutrient lock-out? How can a grower avoid nutrient lock-out?
23. How would you alleviate their concerns with regard to your work and agricultural science as a whole?
24. Can you take us through, step by step, how a grower should approach developing their own plant nutrition program?
25. I’ve been reading about this phenomenon of growers wanting “white ash” from smoking their flower. Why is that and what does it indicate, if anything?
26. Are there any new projects you’re working on that you’d like to talk about?
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